Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar is used for the selective isolation of Vibrio cholerae and other enteropathogenic vibrios according to ISO 21872-1:2017 and ISO 21872-2:2007. Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar is not intended for use in the diagnosis of disease or other conditions in humans.TCBS Agar is also called Vibrio Selective Agar, and prepared according to the formula of Kobayashi et al. This formula is a modification of the selective medium from Nakanishi. All pathogenic Vibrio spp., except Vibrio hollisae, will grow on TCBS Agar. This highly selective agar meets the nutritional requirements of Vibrio spp. Vibrio spp. are able to grow in media containing increased salt concentrations, and some species are halophilic. Infections have been associated with ingestion of contaminated water and consumption of contaminated shellfish or seafood. Vibrio spp. are natural inhabitants of seawater. According to ISO 21872-2, pre-incubation in Alkaline Saline Peptone Water (ASPW) (FSA1548) is required.

• Analyte: Vibrio spp.
• Platform: dehydrated culture media
• Formula: yeast extract 5.0g/L, enzymatic digest of casein 5.0g/L, enzymatic digest of animal tissue 5.0g/L, sodium citrate 10.0g/L, sodium thiosulfate 10.0g/L, bile salts 8.0g/L, sucrose 20.0g/L, sodium chloride 10.0g/L, ferric citrate 1.0g/L, bromothymol blue 0.04g/L, thymol blue 0.04g/L, agar 14.0g/L
• Grams per litre: 88.00
• Litres per 500g: 6.00